Screening and biocontrol evaluation of indigenous native Trichoderma spp. against early blight disease and their field assessment to alleviate natural infection

Abstract Background Early blight disease of tomato caused by pathogenic fungi Alternaria solani is the most significant and common throughout world as well in Kingdom Saudi Arabia. The aim this study was to isolate identify native Trichoderma species from Jeddah region Arabia; evaluate their antagonistic potential against A. ; influence early severity greenhouse open field. Results present focused explore biocontrolling spp. strain compare with a conventional fungicide . Out 21, 3 isolates showed an antifungal activity significantly inhibited mycelial growth pathogen that were identified atroviride, T. harzianum longibrachiatum ITS sequence analysis. Strong vitro suppression (70.66%) also recorded at 400 ppm Mancozeb (90%WP ® ) fungicide. Further, these bioagents further evaluated (artificially inoculated) field on naturally infected plants. In greenhouse, (13.74%) after treatment recorded, followed (25.83%) atroviride (21.67%). (50 mg/L; 10 ml per plant) application (7.91%). positive impact plant biomarkers demonstrated all selected greenhouse. Under natural infection season I, (%) , treatments 11.5, 13.26 16.81%, respectively, control (32.12%), whereas 7.18% application. Conclusions results revealed had mitigate intensity